First Detection of Shv-Type Extended Spectrum Β-Lactamases in The University Hospital Complex Paediatric Charles De Gaulle (CHUP-CDG) of Ouagadougou in Burkina Faso
Strains were isolated from urines clinical samples from patients from various service units of University Hospital complex Paediatric Charles De Gaulle (CHUP-CDG) in Burkina Faso. These strains were resistant to at least one oxyimino-cephalosporin. They were identified as producer of extended spectrum β-lactamases (ESBL) by double-disk synergy test between amoxicillin?clavulanate and cefotaxime, ceftriaxone or ceftazidime and by kinetic methods. Crude extract from these isolates showed a broad-substrate profile by hydrolyzing cefuroxime, cefotaxime, ceftriaxone and with a lower degree to cetazidime and cefepime but not imipinem. Sequencing of PCR products amplified with primers designed for blaSHV genes showed that these genes coded for SHV-11, SHV-12, SHV-28, SHV-32, SHV-38, SHV-76 and SHV-99. This is the first description of these enzymes in Burkina Faso.
Year of publication: |
2014
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Authors: | Amana, METUOR DABIRE ; Jacob, ZONGO K ; Boukaré, ZEBA ; Jihad, MOUSSAWI ; Marie, BAUCHER ; Mondher, EL JAZIRI |
Published in: |
Journal of Asian Scientific Research. - Asian Economic and Social Society. - Vol. 4.2014, 5, p. 214-221
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Publisher: |
Asian Economic and Social Society |
Subject: | SHV-type extended spectrum β-lactamases | University hospital complex paediatric charles de gaulle |
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