Li, Linzhi; Liu, Xing; Su, Benchao; Zhang, Huiyin; Li, Rui - 2022
A novel electrochemical immunoassay based on nanobody heptamer for ultrasensitive detection of alpha fetoprotein (AFP) is proposed. First, AFP heptamer fusion protein (A1-C4bpa) is prepared via fusing the AFP-specific nanobody (A1) with the C-terminal fragment of C4-binding protein (C4bpa). Then...