This Test Guideline describes a method to assess bioaccumulation of sediment-associated chemicals in endobenthic oligochaetes worms. It applies to stable, neutral organic chemicals having log Kow values between 3.0 and 6.0, superlipophilic substances that show a log Kow of more than 6.0, or...

The Hyalella azteca Bioconcentration Test (HYBIT) provides a non-vertebrate test for bioconcentration in aquatic environments. The test consists of two phases: the exposure (uptake) and post-exposure (depuration) phases. During the uptake phase, a group of H. azteca is exposed to the test...

This Test Guideline describes a method to assess the extent and kinetics of primary and ultimate biodegradation of organic chemicals whose route of entry into the environment begins with their discharge to wastewater. It consists of five simulation tests in a) sewer systems, b) activated sludge,...

The purpose of this test is to measure the time course of biodegradation of a test substance at low concentration in aerobic natural water and to quantify the observations in the form of kinetic rate expressions. The test is performed in batch by “pelagic test” or “suspended sediment...

This Test Guideline describes the modified MITI test (II). This test permits the measurement of the Biochemical Oxygen Demand (BOD) and the analysis of residual chemicals in order to evaluate the inherent biodegradability of chemical substances which have been found by the Standard MITI Method...

This Test Guideline describes two simulations tests used in the aerobic sewage treatment. Activated Sludge Units are designed to determine the elimination and the primary and/or ultimate biodegradation of water-soluble organic compounds by aerobic micro-organisms in a continuously operated test...

This Test Guideline is a screening method for the evaluation of ready biodegradability of chemical. The test substance, normally at 20 mg C/L, as the sole source of carbon and energy, is incubated (during 28 days normally) in sealed bottles with aerobic condition containing a buffer-mineral...

This Test Guideline describes a procedure for characterising the bioconcentration potential of substances in fish, under flow-through conditions (but semi-static regimes are permissible). The test consists of two phases: the exposure (uptake) and post-exposure (depuration) phases. During the...

This Test Guideline describes the Zahn-Wellens/EMPA Test. It is used to determine inherent biodegradability. A mixture containing the non-volatile and water soluble test substance, mineral nutrients and a relatively large amount of activated sludge in aqueous medium is agitated and aerated at...

The method described in this Test Guideline is designed for the evaluation of the mineralisation rate of a 14C-labelled compound in soil. The method is applicable to volatile or non-volatile, soluble or insoluble compounds which are not inhibitory to micro-organisms. The basic test consists on...