Bioprocessing Technology for Production of Biopharmaceuticals and Bioproducts

Cover -- Title Page -- Copyright -- Contents -- List of Contributors -- Part I Case Study -- Chapter 1 Bacillus and the Story of Protein Secretion and Production -- 1.1 Bacillus as a Production Host: Introduction and Historical Account -- 1.2 The Building of a Production Strain: Genetic Tools for B. subtilis Manipulation -- 1.2.1 Promoters -- 1.2.2 Vectors for Building a Production Strain -- 1.2.3 B. subtilis Competent Cell Transformation -- 1.2.4 Protoplasts‐Mediated Manipulations -- 1.2.5 Genetics by Electroporation -- 1.3 B. subtilis Secretion System and Heterologous Protein Production -- 1.3.1 Bacillus Fermentation and Recovery of Industrial Enzyme -- 1.3.2 Fermentation Stoichiometry -- 1.3.3 Fermentor Kinetics and Outputs -- 1.3.4 Downstream Processing -- 1.4 Summary -- References -- Chapter 2 New Expression Systems for GPCRs -- 2.1 Introduction -- 2.2 Recombinant GPCR Production - Traditional Approaches for Achieving High‐Level Production -- 2.3 Engineered Expression Systems for GPCR Production -- 2.3.1 Bacteria -- 2.3.2 Yeasts -- 2.3.3 Insect Cells -- 2.3.4 Mammalian Cells -- 2.3.5 Transgenic Animals -- 2.3.6 Cell‐Free Systems -- 2.4 Conclusion -- References -- Chapter 3 Glycosylation -- 3.1 Introduction -- 3.2 Types of Glycosylation -- 3.2.1 N‐linked Glycans -- 3.2.2 O‐linked Glycans -- 3.3 Factors Affecting Glycosylation -- 3.3.1 Nutrient Depletion -- 3.3.2 Fed‐batch Cultures and Supplements -- 3.3.3 Specific Culture Supplements -- 3.3.4 Ammonia -- 3.3.5 pH -- 3.3.6 Oxygen -- 3.3.7 Host Cell Systems -- 3.3.8 Other Factors -- 3.4 Modification of Glycosylation -- 3.4.1 siRNA and Gene Knockout/Knockin -- 3.4.2 Glycoprotein Processing Inhibitors and In Vitro Modification of Glycans -- 3.5 Glycosylation Analysis -- 3.5.1 Release of Glycans from Glycoproteins -- 3.5.2 Derivatization of Glycans -- 3.6 Methods of Analysis -- 3.6.1 Lectin Arrays