Effect of Acyl Acceptor on Biodiesel Production from Soybean Cooking Oil Waste Using Immobilized Aspergillus Oryzae Lipase on Glyoxyl-Agarose Support and Characterization of the Produced Biodiesel
The toxic nature of acyl acceptors to biocatalyst is a major concern for an efficient transesterification process. In this study, bio-esterification of Soybean Cooking Oil Waste (SCOW) for biodiesel production using free and glyoxyl-agarose immobilized lipases from Aspergillus oryzae and the effects of acyl acceptor, molar ratio of methanol to SCOW, and fed-batch addition of methanol on biodiesel production were investigated. The Fatty Acid Methyl Esters (FAMEs) produced were characterized using FTIR, 1 H-NMR, 13 C-NMR and GC-MS. The physico-chemical characterizations of the FAMEs were determined and compared with ASTM and EU standards. Glyoxyl-agarose immobilized lipase (97.7±4.89 a (%)) supported higher biodiesel production as compared to free lipase (87.8±4.39 b (%)). Methanol and methyl ethanoate, molar ratio 1:5, three-fed batch addition of methanol supported the highest biodiesel production. The kinematic viscosity (4.539 cSt), density (0.885), flash point (254 o C), and acid-value (0.266 mg KOH/g) of the produced biodiesel (B100) and the blends were within the accepted limits of international standards. This study presents a cheaper alternative to producing sustainable biodiesel through the use of SCOW. The immobilized lipase and fed-batch addition of methanol had a significant effect on the produced FAME
Year of publication: |
[2022]
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Authors: | Adebami, Gboyega E. ; Adebayo-Tayo, Bukola C. ; Kuila, Arindam |
Publisher: |
[S.l.] : SSRN |
Subject: | Biokraftstoff | Biofuel | Sojabohne | Soybean |
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