In the bioethanol production process, as much as 90% of waste was produced. The availability of waste production is very important since waste can be processed to become livestock feed. The solid bioethanol waste contains cyanide (HCN) 5.8177 mg/kg, water 95,21%, ash 0,39%, protein 8,16%, crude fiber 5,45%, crude fat 2.06%, and carbohydrates 83,94%. Processing bioethanol solid waste into livestock feed can be done by utilizing the existing fungi on bioethanol solid waste. Crude fiber (cellulose) and carbohydrates are a source of cellulolytic fungi. Cellulolytic fungi can degrade the role of organic materials contained in bioethanol solid waste, so that it can be made as a source of highly nutritious livestock feed. This study aims to determine the types of cellulolytic fungal isolates contained in bioethanol solid waste which is potentially processed to become livestock woof. Descriptive analysis was employed as a method of the study. Furthermore, Potato Dextrose Agar (PDA) was used as a medium for culturing and isolating the fungus. Dilution series and pour plate method were employed to isolate the fungus. And, Moist Chamber method was employed to identify it. In addition, Carboxy Methyl Cellulose (CMC) was used as medium to identify cellulolytic fungi. The process was carried out up to the level of genus based on macroscopic and microscopic characterization. 10 fungal isolates from the genus of Aspergillus sp 1, Aspergillus sp 2, Aspergillus sp 3, Aspergillus niger, Cladosporium sp, Mucor sp, Penicillium sp 1, Penicillium sp 2, Rhizopus sp and Trichoderma viride were yielded in this study. The results of examining cellulose enzyme activity revealed that 9 of 10 isolates of the fungus were capable of degrading cellulose. Isolates yielding the largest cellulose enzyme were Trichoderma viride, Penicillium sp 1, Cladosporium sp and Aspergillus niger.
