Recombinant multi-functional cellulase activity in submerged fermentation of lignocellulosic wastes
The expression of the multi-functional cellulase gene mfc in Coprinopsis cinerea is modulated using glyceraldehyde-3-phosphate dehydrogenase (gpd) promoter fragments from Agaricus bisporus, Flammulina velutipes, and Lentinula edodes. In submerged fermentation, with banana peels as the growth substrate, highest activities of endo-β-1,4-glucanase (0.48 U/ml), total cellulase (0.26 U/ml) and endo-β-1,4-xylanase (38.10 U/ml) were noted in the culture liquid Cabm44, which uses a 275 bp gpd fragment from A. bisporus. The lignocellulolytic enzymatic activities were increased nearly twofold in comparison with the wild-type strain.
Year of publication: |
2011
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Authors: | Yang, Peizhou ; Guo, Liqiong ; Cheng, Shujie ; Lou, Nannan ; Lin, Junfang |
Published in: |
Renewable Energy. - Elsevier, ISSN 0960-1481. - Vol. 36.2011, 12, p. 3268-3272
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Publisher: |
Elsevier |
Subject: | Multi-functional cellulase | Coprinopsis cinerea | gpd promoters | Lignocellulose fermentation |
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