This study describes algal biofuel production, bioelectricity generation and wastewater treatment using Leptolyngbya sp. JPMTW1 (KF977831). The experiments were conducted in an AMFC (Algal Microbial Fuel Cell) photobioreactor (single-chamber). The pH, EC (electrical conductivity), COD (chemical oxygen demand) and TDS (total dissolved solids) decreased from 8.01 to 7.0, 982 to 854 (mS/cm), 255 to 112 (mg/L) and 490–427 (mg/L), respectively, over course of 7 days. Biomass production, rate of biomass production, chlorophyll a, b and “total chlorophyll” content increased with increasing time and were observed to be 3300 mg/L, 471.42 mg/L/day, 0.981 mg/L, 0.173 mg/L and 1.156 mg/L after 7 days. Lipid production and rate of lipid production were 1068.383 mg/g dry wt. biomass and 152.62 mg/g dry biomass/day. FTIR (Fourier transform infrared) spectra revealed the presence of protein, lipid, FAs (fatty acids), triglycerides and ester functional groups. FAME (fatty acid methyl esters) profile revealed the presence of C16:0, C18:2n-6, C18:1 and C16:1. The generation of electric potential by Leptolyngbya sp. JPMTW1 increased significantly (p ≤ 0.05) from 0.0211 to 0.264 mV within 7 days. The maximum power density (0.008 mW/cm2) was obtained at cell potential at 12 mV. This study shows that simultaneous production of biofuel, bioelectricity and wastewater treatment is possible by Leptolyngbya sp. JPMTW1.
